Mario is one of the eight playable characters in Mario + Rabbids Kingdom Battle, ​and is one of the three starting characters in the game, alongside Rabbid Peach and Rabbid Luigi. In the game, Mario teams up with Rabbid Peach, Rabbid Luigi, Luigi, Rabbid Mario, Peach, Rabbid Yoshi and Yoshi to save the Mushroom Kingdom from the mischievous Rabbids.

Mario is described as a Team Fighter, being an all-around hero with good offense, defense and mobility. Unlike all other heroes, Mario cannot be swapped out from the team for another hero, meaning that Mario will always be a member of your team, which is good due to his all-around play style. His primary weapon is a Blaster, which has decent damage and range, however is mainly good at close and mid-ranges, and his secondary weapon is Melee, allowing Mario to hit nearby enemies with his Hammer for huge damage. Unlike other heroes, Mario has the ability to 'Stomp Jump' enemies by Team Jumping off an ally, then stomping on an enemy to deal extra damage to them. His special abilities include Hero Sight, allowing Mario to attack an enemy on their turn if they move out of cover and if they are in Mario's range, and M-Power, allowing Mario to give a 50% damage boost to all heroes in his range, including himself. His M-power can be used with his Rabbid counterpart's Boom Dash to increase damage towards enemies and Mario's Hero Sight can be used with forced movement abilities(e.g. Rabbid Yoshi's Scaredy Rabbid).His M-power can also be used with Yoshi's Super Chance to deal even more weapon damage. His Stomp jump can also be used with his M-power ability to deal even more movement damage.

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