Bwario appears in the Lava Pits, summoned by the MegaBug to stop the heroes from reaching Bowser's Castle. He is fought twice in the game; the first time alongside his stronger ally Bwaluigi, and the second time alongside both Bwaluigi and the Lava Queen. Bwario behaves similarly to a Valkyrie. He has significantly less HP than Bwaluigi, but attacks at range with a machine gun seemingly built out of green pipes. He can also activate a "line of sight" technique to hit heroes as they move, which works nicely with Bwaluigi and Lava Queen's forced movement abilities. His team jumps also produce close-range shockwaves upon landing, seemingly by smashing the ground with his gun. He and all of his allies must be defeated in order to claim victory.